The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world
Identifieur interne : 001430 ( Pmc/Checkpoint ); précédent : 001429; suivant : 001431The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world
Auteurs : Marie-Pierre Egloff [France] ; François Ferron [France] ; Valérie Campanacci [France] ; Sonia Longhi [France] ; Corinne Rancurel [France] ; Hélène Dutartre [France] ; Eric J. Snijder [Pays-Bas] ; Alexander E. Gorbalenya [Pays-Bas] ; Christian Cambillau [France] ; Bruno Canard [France]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2004.
Abstract
The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to
Url:
DOI: 10.1073/pnas.0307877101
PubMed: 15007178
PubMed Central: 374323
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world</title><author><name sortKey="Egloff, Marie Pierre" sort="Egloff, Marie Pierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Dutartre, Helene" sort="Dutartre, Helene" uniqKey="Dutartre H" first="Hélène" last="Dutartre">Hélène Dutartre</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name><affiliation wicri:level="3"><nlm:aff id="N0x8a583c0.0x97d42d8">Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Gorbalenya, Alexander E" sort="Gorbalenya, Alexander E" uniqKey="Gorbalenya A" first="Alexander E." last="Gorbalenya">Alexander E. Gorbalenya</name><affiliation wicri:level="3"><nlm:aff id="N0x8a583c0.0x97d42d8">Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15007178</idno><idno type="pmc">374323</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC374323</idno><idno type="RBID">PMC:374323</idno><idno type="doi">10.1073/pnas.0307877101</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000743</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000743</idno><idno type="wicri:Area/Pmc/Curation">000743</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000743</idno><idno type="wicri:Area/Pmc/Checkpoint">001430</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001430</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world</title><author><name sortKey="Egloff, Marie Pierre" sort="Egloff, Marie Pierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Dutartre, Helene" sort="Dutartre, Helene" uniqKey="Dutartre H" first="Hélène" last="Dutartre">Hélène Dutartre</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name><affiliation wicri:level="3"><nlm:aff id="N0x8a583c0.0x97d42d8">Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Gorbalenya, Alexander E" sort="Gorbalenya, Alexander E" uniqKey="Gorbalenya A" first="Alexander E." last="Gorbalenya">Alexander E. Gorbalenya</name><affiliation wicri:level="3"><nlm:aff id="N0x8a583c0.0x97d42d8">Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="N0x8a583c0.0x97d42d8">Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to <italic>Coronaviridae</italic> (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we report the crystal structure at 2.7-Å resolution of nsp9, a hitherto uncharacterized subunit of the SARS-CoV replicative polyproteins. We show that SARS-CoV nsp9 is a single-stranded RNA-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. The presence of this type of protein has not been detected in the replicative complexes of RNA viruses, and its presence may reflect the unique and complex CoV viral replication/transcription machinery.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-id journal-id-type="publisher-id">pnas</journal-id><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn><publisher><publisher-name>National Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">15007178</article-id><article-id pub-id-type="pmc">374323</article-id><article-id pub-id-type="publisher-id">1013792</article-id><article-id pub-id-type="doi">10.1073/pnas.0307877101</article-id><article-categories><subj-group subj-group-type="heading"><subject>Biological Sciences</subject><subj-group><subject>Biochemistry</subject></subj-group></subj-group></article-categories><title-group><article-title>The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Egloff</surname><given-names>Marie-Pierre</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref></contrib><contrib contrib-type="author"><name><surname>Ferron</surname><given-names>François</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref></contrib><contrib contrib-type="author"><name><surname>Campanacci</surname><given-names>Valérie</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref></contrib><contrib contrib-type="author"><name><surname>Longhi</surname><given-names>Sonia</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref></contrib><contrib contrib-type="author"><name><surname>Rancurel</surname><given-names>Corinne</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref></contrib><contrib contrib-type="author"><name><surname>Dutartre</surname><given-names>Hélène</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref></contrib><contrib contrib-type="author"><name><surname>Snijder</surname><given-names>Eric J.</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">†</xref></contrib><contrib contrib-type="author"><name><surname>Gorbalenya</surname><given-names>Alexander E.</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">†</xref></contrib><contrib contrib-type="author"><name><surname>Cambillau</surname><given-names>Christian</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref><xref ref-type="corresp" rid="cor1">‡</xref></contrib><contrib contrib-type="author"><name><surname>Canard</surname><given-names>Bruno</given-names></name><xref ref-type="aff" rid="N0x8a583c0.0x97d42d8">*</xref><xref ref-type="corresp" rid="cor1">‡</xref></contrib></contrib-group><aff id="N0x8a583c0.0x97d42d8"><label>*</label>Architecture et Fonction des Macromolécules Biologiques, Unité Mixte de Recherche 6098 Centre National de la Recherche Scientifique and Universités Aix-Marseille I et II, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France; and<label>†</label>Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands</aff><author-notes><fn id="cor1"><label>‡</label><p> To whom correspondence should be addressed. E-mail: <email>cambillau@afmb.cnrs-mrs.fr</email> or <email>bruno@afmb.cnrs-mrs.fr</email>. </p></fn><fn><p>Edited by Stephen C. Harrison, Harvard Medical School, Boston, MA, and approved January 28, 2004</p></fn></author-notes><pub-date pub-type="ppub"><day>16</day><month>3</month><year>2004</year></pub-date><pub-date pub-type="epub"><day>8</day><month>3</month><year>2004</year></pub-date><volume>101</volume><issue>11</issue><fpage>3792</fpage><lpage>3796</lpage><history><date date-type="received"><day>26</day><month>11</month><year>2003</year></date></history><copyright-statement>Copyright © 2004, The National Academy of Sciences</copyright-statement><copyright-year>2004</copyright-year><abstract><p>The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to <italic>Coronaviridae</italic> (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we report the crystal structure at 2.7-Å resolution of nsp9, a hitherto uncharacterized subunit of the SARS-CoV replicative polyproteins. We show that SARS-CoV nsp9 is a single-stranded RNA-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. The presence of this type of protein has not been detected in the replicative complexes of RNA viruses, and its presence may reflect the unique and complex CoV viral replication/transcription machinery.</p></abstract></article-meta><notes><fn-group><fn><p>This paper was submitted directly (Track II) to the PNAS office.</p></fn><fn><p>Abbreviations: SARS, severe acute respiratory syndrome; CoV, coronavirus; ss, single-stranded; SSB, ssDNA-binding protein; OB, oligosaccharide/oligonucleotide-binding; <italic>K</italic><sub>Dapp</sub>, dissociation equilibrium constant; NCBI, National Center for Biotechnology Information.</p></fn><fn><p>Data deposition: The atomic coordinates and structure factors have been deposited in the Protein Data Bank, <ext-link ext-link-type="uri" xlink:href="www.pdb.org">www.pdb.org</ext-link> (PDB ID code 1QZ8).</p></fn></fn-group></notes></front></pmc><affiliations><list><country><li>France</li><li>Pays-Bas</li></country><region><li>Hollande-Méridionale</li><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Leyde</li><li>Marseille</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Egloff, Marie Pierre" sort="Egloff, Marie Pierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name></region><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Dutartre, Helene" sort="Dutartre, Helene" uniqKey="Dutartre H" first="Hélène" last="Dutartre">Hélène Dutartre</name><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name></country><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name></region><name sortKey="Gorbalenya, Alexander E" sort="Gorbalenya, Alexander E" uniqKey="Gorbalenya A" first="Alexander E." last="Gorbalenya">Alexander E. Gorbalenya</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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